Identification of genes in cassava that are differentially expressed during infection with Xanthomonas axonopodis pv. manihotis
Identifieur interne : 002956 ( Main/Exploration ); précédent : 002955; suivant : 002957Identification of genes in cassava that are differentially expressed during infection with Xanthomonas axonopodis pv. manihotis
Auteurs : Marcella Santaella [Colombie] ; Edna Suárez [Colombie] ; Camilo L Pez [Colombie, France] ; Carolina González [Colombie, France] ; Gloria Mosquera [Colombie] ; Silvia Restrepo [Colombie] ; Joe Tohme [Colombie] ; Alfredo Badillo [Colombie] ; Valérie Verdier [France]Source :
- Molecular Plant Pathology [ 1464-6722 ] ; 2004-11.
Abstract
The cDNA‐amplified fragment length polymorphism approach was used to identify differentially expressed transcripts from cassava infected by Xanthomonas axonopodis pv. manihotis (Xam). Approximately 3600 transcript‐derived fragments (TDFs) were screened of which 340 were isolated. The nucleotide sequences of 250 TDFs were analysed and assembled into contigs and singletons. The amino acid sequences of their predicted products were compared with entries in databases and 63 of these clones showed homology to known plant genes. Of these, 32 showed similarity to plant defence proteins. Fifty‐one TDFs corresponded to proteins of unknown function and 106 did not match any sequence in the public databases. Quantitative reverse transcription PCR was carried out with a selected set of gene transcripts that demonstrated an increase of expression during the infection. These results point out candidate genes that are associated with cassava resistance to Xam and reinforce the idea of a complex process occurring during this plant–pathogen interaction.
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DOI: 10.1111/j.1364-3703.2004.00254.x
Affiliations:
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Approximately 3600 transcript‐derived fragments (TDFs) were screened of which 340 were isolated. The nucleotide sequences of 250 TDFs were analysed and assembled into contigs and singletons. The amino acid sequences of their predicted products were compared with entries in databases and 63 of these clones showed homology to known plant genes. Of these, 32 showed similarity to plant defence proteins. Fifty‐one TDFs corresponded to proteins of unknown function and 106 did not match any sequence in the public databases. Quantitative reverse transcription PCR was carried out with a selected set of gene transcripts that demonstrated an increase of expression during the infection. These results point out candidate genes that are associated with cassava resistance to Xam and reinforce the idea of a complex process occurring during this plant–pathogen interaction.</div></front></TEI><affiliations><list><country><li>Colombie</li><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Perpignan</li></settlement></list><tree><country name="Colombie"><noRegion><name sortKey="Santaella, Marcella" sort="Santaella, Marcella" uniqKey="Santaella M" first="Marcella" last="Santaella">Marcella Santaella</name></noRegion><name sortKey="Badillo, Alfredo" sort="Badillo, Alfredo" uniqKey="Badillo A" first="Alfredo" last="Badillo">Alfredo Badillo</name><name sortKey="Gonzalez, Carolina" sort="Gonzalez, Carolina" uniqKey="Gonzalez C" first="Carolina" last="González">Carolina González</name><name sortKey="L Pez, Camilo" sort="L Pez, Camilo" uniqKey="L Pez C" first="Camilo" last="L Pez">Camilo L Pez</name><name sortKey="Mosquera, Gloria" sort="Mosquera, Gloria" uniqKey="Mosquera G" first="Gloria" last="Mosquera">Gloria Mosquera</name><name sortKey="Restrepo, Silvia" sort="Restrepo, Silvia" uniqKey="Restrepo S" first="Silvia" last="Restrepo">Silvia Restrepo</name><name sortKey="Suarez, Edna" sort="Suarez, Edna" uniqKey="Suarez E" first="Edna" last="Suárez">Edna Suárez</name><name sortKey="Tohme, Joe" sort="Tohme, Joe" uniqKey="Tohme J" first="Joe" last="Tohme">Joe Tohme</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="L Pez, Camilo" sort="L Pez, Camilo" uniqKey="L Pez C" first="Camilo" last="L Pez">Camilo L Pez</name></region><name sortKey="Gonzalez, Carolina" sort="Gonzalez, Carolina" uniqKey="Gonzalez C" first="Carolina" last="González">Carolina González</name><name sortKey="Verdier, Valerie" sort="Verdier, Valerie" uniqKey="Verdier V" first="Valérie" last="Verdier">Valérie Verdier</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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